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Latest Posts by Phil Hugenholtz

Our work on 'hidden diversity' in unbinned contigs is now published in @natmicrobiol.nature.com :

www.nature.com/articles/s41...

See the linked threads for more details!

5 days ago 61 37 3 1
MBE Call for Papers on the Major Transitions of Life

MBE Call for Papers on the Major Transitions of Life

MBE is excited to launch our newest Call for Papers on the Major Transitions of Life, covering all aspects of phylogenomic research.

🔗 academic.oup.com/mbe/pages/call-for-papers-on-the-major-transitions-of-life

Guest Editors:
Davide Pisani
Anja Spang

#evobio #molbio #phylogenetics

5 days ago 22 13 1 0
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Micro-scale spatial metagenomics opens a new era in microbiome ecology Understanding microbial communities requires moving beyond 2D representations toward a holistic view that couples 3D spatial organization with ecological function, integrating microbial inventories, g...

Micro-scale spatial metagenomics opens a new era in microbiome ecology

@cp-trendsmicrobiol.bsky.social Opinion from @mizrahilab.bsky.social

www.cell.com/trends/micro...

5 days ago 22 11 2 1

Metagenomic mining reveals extensive novelty, enhanced biodegradation potential, and untapped biosynthetic capacity in Chinese oilfield microbiomes journals.asm.org/doi/full/10.... #jcampubs

5 days ago 6 2 0 0
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Redox chemistry of early Earth and the origin of life - Communications Chemistry Redox reactions played an important role in shaping the conditions that enabled the emergence of life on the early Earth, but how these relate to specific environmental conditions and whether they led...

Redox chemistry of early Earth and the origin of life www.nature.com/articles/s42... #jcampubs

5 days ago 32 15 0 1
Figure 1 shows the conceptual workflow of the algorithm. It first loads the configuration file and the input data of one or multiple binned metagenomes, yet evaluates each bin individually. It then iteratively processes each candidate bin in the input, going through the refinement tasks, potentially multiple times. The refinement either stops when the last step or a preset stop task have been reached or an unrecoverable error has been encountered. The algorithm tasks can be configured individually.

Figure 1 shows the conceptual workflow of the algorithm. It first loads the configuration file and the input data of one or multiple binned metagenomes, yet evaluates each bin individually. It then iteratively processes each candidate bin in the input, going through the refinement tasks, potentially multiple times. The refinement either stops when the last step or a preset stop task have been reached or an unrecoverable error has been encountered. The algorithm tasks can be configured individually.

🤯 Manual MAG refinement? Still doing it by hand??

We’ve been there... You could just leave those messy, ill-assigned contigs/scaffolds in your data for NCBI to pick up during your submission…

…OR you could add itBins, our new automated MAG refiner by @jmk-ude.bsky.social, to your pipeline!! 🔥

6 days ago 12 7 1 0
SeqCode Prize | SeqCode Registry

The SeqCode Committee has opened the call for applications to the SeqCode Prize 2026, sponsored by ISME.

@isme-microbes.bsky.social

1 week ago 1 4 1 0
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Myloasm, our long-read metagenome assembler, is now published! w/ @mgmarin.bsky.social and @lh3lh3.bsky.social

Very rewarding after > a year of development and countless hours thinking about assembly. Thanks to beta testers, Li lab, and reviewers who gave very helpful feedback.

rdcu.be/famFj

1 week ago 97 56 4 1
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How much protein diversity can Life on Earth actually generate?

With DIAMOND DeepClust, we show how billions of proteins across the tree of life can be clustered at low-identity for downstream analytics tasks.

📚Paper: www.nature.com/articles/s41...
💻Code: github.com/bbuchfink/di...

2 weeks ago 64 29 1 0
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Aerobic sister lineage of breviates has gene-rich mitochondrial genomes Cho et al. describe a novel, aerobic sister lineage of Breviatea using flow cytometric single-cell sorting and genome amplification. The new lineage harbors gene-rich mitochondrial genomes, unlike Bre...

Aerobic sister lineage of breviates has gene-rich mitochondrial genomes
#microbiology #eukaryotes #mitochondria #protists #MicroSky #ProtistsOnSky
@cellpress.bsky.social
www.cell.com/current-biol...

2 weeks ago 12 8 1 0

New paper in mSystems! 🧵 - how much of your metagenome is actually bacterial/archaeal DNA? For many samples, nobody knows.

We built SingleM prokaryotic_fraction (SPF) to answer this, then ran it on >100,000 public metagenomes. 🧬🖥️🦠

Here's what we found 👇
doi.org/10.1128/msystems.01062-25

2 weeks ago 46 28 1 2
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A novel planctomycetotal isolate from subsurface percolates belongs to the novel species Anatilimnocola aquadivae sp. nov. in the family Pirellulaceae
#microbiology #bacteria #NewSpecies #taxonomy #MicroSky
doi.org/10.1038/s415...

2 weeks ago 6 2 1 0
Image of the cover of the 1st volume of ISME Host Microbe a new official journal of the International Society for Microbial Ecology. Cover images shows a plant stomata with bacteria around and entering.

Image of the cover of the 1st volume of ISME Host Microbe a new official journal of the International Society for Microbial Ecology. Cover images shows a plant stomata with bacteria around and entering.

Excited to announce our new journal from @isme-microbes.bsky.social ISME Host Microbe is live and now accepting submissions. Look forward to receiving your papers! #Microsky 🧫 🦠

2 weeks ago 119 82 3 1
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Automated genome mining predicts structural diversity and taxonomic distribution of peptide metallophores across bacteria Automated detection of metallophore biosynthesis reveals that metal-chelating non-ribosomal peptides are widespread, chemically diverse, and deeply rooted in bacterial evolution.

Are you interested in how to predict functions of natural product biosynthetic gene clusters (BGCs) and their products? And/or do you love metallophores and would love to identify their producers in microbiomes? Check out @zachreitz.bsky.social 's new paper!
elifesciences.org/articles/109... 1/n

4 weeks ago 27 13 4 1

The recovery of 12,789 genomes revealed the diversity, function, and microbial interactions of the geothermal spring microbiome www.biorxiv.org/content/10.64898/2026.03...

4 weeks ago 3 2 0 0

A very good likeness!

4 weeks ago 0 0 0 0
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Genomic insights into adaptation and microevolution of two novel non-AOA #Nitrososphaeria, Acidarchaeum fankouense and Thermosulfuris yongpingense, in acid mine drainage ecosystems www.sciencedirect.com/science/arti...

4 weeks ago 5 4 0 0
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Oops, typo in the family name in the abstract

4 weeks ago 0 0 0 0
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Apolloniradiicaulis salifontis gen. nov., sp. nov., a New Prosthecate Aerobic Anoxygenic Phototroph Isolated from Lake Winnipegosis Region Salt Springs
#microbiology #NewSpecies #taxonomy #MicroSky
doi.org/10.3390/micr...

4 weeks ago 3 2 2 0
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Thermophilic bacteria employ a contractile injection system in hot spring microbial mats Abstract. Bacterial contractile injection systems (CISs) are multiprotein complexes that facilitate the bacterial response to environmental factors or inte

Thermophilic bacteria employ a contractile injection system in hot spring microbial mats
#microbiology #thermophiles #bacteria #MicroSky
@isme-microbes.bsky.social
doi.org/10.1093/isme...

4 weeks ago 9 4 1 0
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Diversity, ecology, cell biology and evolution of the Asgard archaea Nature Reviews Microbiology, Published online: 05 March 2026; doi:10.1038/s41579-026-01288-wThe Asgard archaea have become a cornerstone of archaeal research, particularly for studies aiming to unravel the origin and early evolution of eukaryotes. This Review outlines the current state of the research field focusing on these intriguing microorganisms and discusses future research directions aiming to resolve their diversity, ecology, cell biology and evolution.

New online! Diversity, ecology, cell biology and evolution of the Asgard archaea

1 month ago 13 4 0 0
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Phenogenomics reveals the ecology and evolution of Trichoderma fungi for sustainable agriculture - Nature Microbiology Analysis of 37 genomes together with more than 140 phenotypic traits links genomic features to ecological fitness and lifestyle diversity in Trichoderma fungi.

Phenogenomics reveals the ecology and evolution of Trichoderma fungi for sustainable agriculture

-in @natmicrobiol.nature.com by Andrei Steindorff (@jgi.doe.gov) et al from Irina Druzhinina (at @rbgkew.bsky.social), Feng Cai, Igor Grigoriev, and a large network

www.nature.com/articles/s41...

1 month ago 9 5 0 0
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With Eugene Koonin, we propose a concept of “the selfish ribosome”, under which evolution of life is viewed as a ribosomal takeover, where the ribosome evolved to consume most of the cell’s resources, while other cellular componentry ensures the propagation of the ribosome. arxiv.org/abs/2602.23268

1 month ago 236 103 5 14

Calibrating for absolute microbiome abundances without spike-ins www.biorxiv.org/content/10.6... #jcampubs

1 month ago 8 4 1 0
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I have the same cat

1 month ago 2 1 0 0
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Huge news 🌍🧬
The Protist 10,000 Genomes Project is joining the Earth BioGenome Project.
Protists may represent the majority of eukaryotic diversity — yet only a tiny fraction have reference genomes.
Time to change that. 🚀✨
Learn more 👉 ngdc.cncb.ac.cn/p10k/
#EBP #Protists #Genomics #TreeOfLife

1 month ago 49 19 0 0

Metax looks really nice for taxonomic profiling: www.biorxiv.org/content/10.6...

1 month ago 10 5 0 0
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Beyond thrilled to share that our study has been published!
This project encompasses years of work, including my thesis research on Asgard archaea in the @archaeal.bsky.social lab at @utmsi.bsky.social and
@texasscience.bsky.social!!!
#MicroSky #ArchaeaSky 1/12

1 month ago 69 30 8 2
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Oxygen metabolism in descendants of the archaeal-eukaryotic ancestor - Nature Sequencing of marine sediments finds 136 newly identified Heimdallarchaeia and several novel lineages, and indicates that Heimdallarchaeia evolved distinct metabolic capabilities from other Asgar...

#NatMicroPicks

Aerobic archaeal-eukaryotic ancestor? 🦠🫁

The ancestor of eukaryotes may have combined hydrogen metabolism with aerobic respiration, shaping early cellular complexity.

#MicroSky

www.nature.com/articles/s41...

1 month ago 34 18 0 0
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Robust estimation of microbial diversity in theory and in practice AbstractQuantifying diversity is of central importance for the study of structure, function and evolution of microbial communities. The estimation of micro

hey @joshuasweitz.bsky.social this paper rocks. empirical confirmation of an intuition that is currently load-bearing in a paper i've been scared to finish. i love it academic.oup.com/ismej/articl...

1 month ago 18 2 2 0