![Figure showing experimental set-up for ototoxicity study, demonstrating that vibratome sectioning preserves otic vesicles. Panel A illustrates vibratome sectioning of day‑75 IEOs into ~200 µm slices, followed by 48 h recovery and 24 h drug treatment at day 77. Sections were fixed were fixed at 1, 3 or 7 days after treatment. (B) Side-view representative image of an IEO sectioned into 200 µm-thick slices. (C) Top-view representation of a vibratome slice of an IEO, with arrowheads annotating the otic vesicles (inset). Scale bars: 100 µm and 500 µm. (D) Intact morphology of an otic vesicle without vibratoming (I), directly after vibratoming (II) and after 48 h recovery (III), showing Hematoxylin and Eosin (H&E) histological staining, followed by immunofluorescent staining of otic epithelium [CDH1+ (green)/SOX10+ (cyan)] and of otic vesicles containing hair cells (MYO7A+, yellow), neurons (TUBB3+, magenta) and cell nuclei (DAPI+, dark blue). In III, the apparent MYO7A–TUBB3 colocalization likely reflects immature hair cell precursors that transiently co-express neuronal β-III-tubulin during development. Scale bars: 25 µm.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:5jupfg23qpjk2643o6aeftly/bafkreihvdypnftitckmausztj6bja4jcygx5ypm4znh7354d2mhgzsbri4)
Figure showing experimental set-up for ototoxicity study, demonstrating that vibratome sectioning preserves otic vesicles. Panel A illustrates vibratome sectioning of day‑75 IEOs into ~200 µm slices, followed by 48 h recovery and 24 h drug treatment at day 77. Sections were fixed were fixed at 1, 3 or 7 days after treatment. (B) Side-view representative image of an IEO sectioned into 200 µm-thick slices. (C) Top-view representation of a vibratome slice of an IEO, with arrowheads annotating the otic vesicles (inset). Scale bars: 100 µm and 500 µm. (D) Intact morphology of an otic vesicle without vibratoming (I), directly after vibratoming (II) and after 48 h recovery (III), showing Hematoxylin and Eosin (H&E) histological staining, followed by immunofluorescent staining of otic epithelium [CDH1+ (green)/SOX10+ (cyan)] and of otic vesicles containing hair cells (MYO7A+, yellow), neurons (TUBB3+, magenta) and cell nuclei (DAPI+, dark blue). In III, the apparent MYO7A–TUBB3 colocalization likely reflects immature hair cell precursors that transiently co-express neuronal β-III-tubulin during development. Scale bars: 25 µm.
In a new paper in our #InVitroModelling Special Issue, @amylucassen.bsky.social, Heiko Locher & co develop human #iPSC derived inner ear #organoids that reveal ototoxicity in response to cisplatin and gentamicin
doi.org/10.1242/dmm....
