🌿 First #kenaf (Hibiscus cannabinus) #CRISPR system established
K599 hairy roots (cotyledon petiole): ~100% transformation
Optimized: OD₆₀₀=1.0, 20 min infection, 22°C co-cultivation
HcCLA1 dual sgRNA: 41.2% editing (14/34 T-DNA+ lines, GhU6.7 promoter)
GhU6.9: 9.7% (4.2× lower)