Latest posts tagged with #EndothelialCell on Bluesky
AngioTag
#Zebrafish kdrl+ #EndothelialCell RiboTag + TRAP-seq track endothelial translation activity in vivo
Discover novel EC functional genes😎
Profile Orangotypic endothelial features in🐟
Also UAS::RiboTag for any🐟tissues
Brant Weinstein lab #Angiogenesis 2025
link.springer.com/article/10.1...
#ThrowbackThursday in JHC: "Role of the Pancreatic Islet Microvasculature in Health and Disease."
Click to read for FREE: bit.ly/4fihNMw
#capillary #diabetes #endothelialcell
#EndothelialCell #MerTK #Efferocytosis #Atherosclerosis
EC MerTK KO🐭+ AAV8-PCSK9 + #HighFatDiet 2-mo
👉⏫lesion burden
👉⏫NADPH oxidase
👉⏫inflamm cytokines
Aortic Arch lesion #Proteomics➡️
⏫TGFβ HIF1α Wnt3a IL2
⏬LARP1 NAMPT TEAD1
Zufeng Ding lab #RedoxBiol 2025
www.sciencedirect.com/science/arti...
#TGF-β1 drives maladaptive #BoneMarrow endothelial repair—disrupting #VascularRegeneration and #hematopoietic support—while TGF-β1 inhibition restores #Endothelialcell function and promotes #multilineage recovery. @pku1898.bsky.social
#FeaturedArticle #STTT: doi.org/10.1038/s413...
Perturbing MEK–ERK–c-Myc bioenergetic axis in tumor #EndothelialCell suppress primary tumor growth
Evidence from an engineered tumor cell type-selective #AnthraxToxin system allowing
MEK cleavage/inactivation in selective #TumorMicroEnvironmental cell types
#PNAS 2025
www.pnas.org/doi/10.1073/...
GPR153-cAMP/CREB-#YAP/TAZ or -NFκB cascades in #EndothelialCell #SmoothMuscleCell
GPR153
⏫in SMC in intima, atheroma, MI ischemic zone
⏫in EC in inflamed SkM, Brain
Myh11+ SMC Gpr153 KO 🐭
Injured artery
Slco1c1+ brain EC Gpr153 KO 🐭
EAE & Stroke
#NatComm 2025
www.nature.com/articles/s41...
BBB on a chip
#EndothelialCell #Pericyte Nanomembrane (100 nm-thick; 3 or 5 μm pore)
EC-derived laminin is insufficient to fill the pores, which can be covered by pericyte co-culture
Does vascular basement membrane have such pores?🤔
#MatTodayBio
www.sciencedirect.com/science/arti...
INduced Pdgfrb+ #Pericyte ITGA8 KO🐭
Baseline by 40 d
⏬microvascular pericytic coverage
⏬ #EndothelialCell #TightJunction
⏬ #BloodBrainBarrier
⏬EC-Pericyte N-cad junction
⏫Degenerated neurons
⏫Neurobehavioral anomalies
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
#Atherosclerosis Epitranscriptomics
Cell type-specific dysregulation of m6A machinery in atherogenic phenotypes
#EndothelialCell ALKBH5
#SmoothMuscleCell WTAP
Mφ METTL3 YTHDF2
Re-analysis of scRNAseq Wirka's Nat Med 2019
pmc.ncbi.nlm.nih.gov/articles/PMC...
#THNO 2025
www.thno.org/v15p4785.htm
#WernerSyndrome hiPSC engineered vessel
Aligned #SmoothMuscleCell in Collagen hydrogel➡️
Roll-up as helical tube
+PLA mold
+Luminal #EndothelialCell
#SpatialTranscriptomics
#Metabolomics
Vessels with Werner EC are Hypercontractile even with healthy SMC
bioRxiv 2025
www.biorxiv.org/content/10.1...
#TissueEngineeredBloodVessel
Decellularized 👤umbilical cord artery
Seeded: #hiPSC #EndothelialCell
Bioreactor trained: pulsatile unidirec flow 15➡️5 dyn/cm2
Rat #InferiorVenaCava graft▶️
0️⃣Thrombosis
Complete repop by host EC 2 wk SMC 2 mo
#CellStemCell 2025
www.sciencedirect.com/science/arti...
A 3D fibrin hydrogel supportive neurovascular cell niche
17 kPa ECM stiffness
👤🧠 #EndothelialCell #Pericyte #Astrocyte
⏫Quiescence, invasiveness, temozolomide resistance of patient-derived #Glioblastoma stem cell lines or tumorsphere
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
#Senescence #Mechanobiology
Senescent #EndothelialCell HUVEC (+H2O2 or Doxorubicin) have ⬆️ #Viscoelasticity
(Microfluidic cytometric measurement of suspended whole-cell deformation)
⏫F-actin, Calnexin ER, Lamin A Nuclear envelope
#CellRepPhysSci 2025
www.sciencedirect.com/science/arti...
#Stroke #Angiogenesis
Post-stroke (7 dpi) KO of #EndothelialCell HDAC2🐭
👉⏫Preserved Cortical volume by 8 wk
👉⏫Neuron survival
👉⏫Angiogensis
👉⏫#BloodBrainBarrier (3 kD dextran)
Kumar Peeyush lab bioRxiv 2025
www.biorxiv.org/content/10.1...
AAV-BI30, an endotheliotropic #AdenoAssociatedVirus for transducing vascular tree in #CentralNervousSystem Brain/Retina/Spinal Cord
👉>AAV-BR1 esp. in artery/vein
👉Also transduce #EndothelialCell in #Aorta/Liver/Lung/Kidney
@natcardiovascres.nature.com 2022
pmc.ncbi.nlm.nih.gov/articles/PMC...
Stroke Atlas
anratherlab.shinyapps.io/strokevis/
Single-cell transcriptomics 🐭Brain+Blood
2 & 14 days post-stroke
♀️♂️
Young(2~3 mo) Aged (17~20 mo)
9 #EndothelialCell clusters
IGF1 signals
#Aging
⏫interferon signals in post-stroke🧠EC (+immune cell)
#NatImmunol 2024
www.nature.com/articles/s41...
Single-cell transcriptomics #SuperficialTemporalArtery #MoyamoyaDisease
2 Case + 3 Ctrl (epilepsy)
SMC1: contractile OXPHOS
SMC2: ribosome
Proliferative signaling
✅#NaturalKiller MIF➡️ SMC Fibroblast
#EndothelialCell
#JAHA 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
#Malaria #BloodBrainBarrier
P. falciparum (most severe human malaria parasite strain)-infected #RedBloodCell➡️
Local disruption of #EndothelialCell barrier in 3D microfluidic BBB (EC Pericyte Astrocyte)
⏫Interferon-JAK-STAT1 signals in all 3 cell types
#NatCommun 2025
www.nature.com/articles/s41...
Endothelin-1➡️ #EndothelialCell to #DorsalRootGanglion signaling in #VascularPain
✅Mechanical ❎Heat ❎Cold
1⃣🐭Limb ischemia
2⃣🐭Optogenetic EC ChR2 nociception
3⃣🐭Chemogenetic EC hM3Dq nociception
4⃣🧍♂️Postop tourniquet pain⬇️by Bosentan ETA/Bi
#AdvSci 2025
advanced.onlinelibrary.wiley.com/doi/10.1002/...
Fabrication of #MicrovesselOnAChip
#EndothelialCell HUVEC
3D printing injection reservoirs
Needle-based collagen channel φ200 μm
Sprouting
100 Pa intraluminal pressure
4 & 70 kD FITC-dextran for permeability
A. Bancaud & Y. Matsunaga labs #STARProtoc 2024
www.sciencedirect.com/science/arti...
triggers inflammation throughout the body by activating immune & #endothelialcells.C5a also promotes the release of pro-inflammatory cytokines like IL-6.
- Vascular Injury: The formation and insertion of TCC into #endothelialcell membranes directly cause cellular injury and death,
Lineage Origins of mouse renal #LymphaticEndothelialCell
85% by Tie2+ Venous #endothelialCell
15% by Osr1+ Intermediate Mesoderm😎
Lymphvasculogenesis (60% Tie2+ vs 40% Osr1+)
9 different Cre🐭🤠
@daniyaljafree.bsky.social bioRxiv 2025
www.biorxiv.org/content/10.1...
Tie2+ #EndothelialCell- or Col1A2+ fibroblast-derived C-type Natriuretic Peptide protects against #AorticAneurysm in Apoe-/- mice + AngII + AAV-PCSK9 + #WesternDiet
#SmoothMuscleCell & Mφ are responsive to exogenous CNP
#ATVB 2025 @ahajournals.bsky.social
www.ahajournals.org/doi/10.1161/...
Anti-isoDGR immunotherapy stabilizes Aged 🐭 #BloodBrainBarrier
#ExtraCellularMatrix Protein Deamidation
Asn-Gly-Arg/NRG➡️isoaspartate-Gly-Arg/isoDGR
⏫in #Aging
isoDGR on #EndothelialCell in vitro
👉Actin cytoskeletal alterations
👉Inflammation
👉Senescence
bioRxiv 2025
www.biorxiv.org/content/10.1...
Serial block-face SEM of mouse Heart Capillaries #EndothelialCell #Pericyte #Telocyte
There are direct physical contacts b/w CD34+/CD44+ perivascular mesenchymal cells & abluminal protrusions of EC!
#Cells_MDPI 2025
www.mdpi.com/2073-4409/14...
Non-hepatic #PCSK9
PCSK9 overexpression regulates #ExtracellularVescle cargo packaging (Proteome microRNA array) in #SmoothMuscleCell▶️
👉⏫Inflammation in #EndothelialCell Mφ
👉⏫Migration ⏫oxLDL uptake ⏬OXPHOS ⏫Glycolysis in Mφ
👉Recruit more Mφ in🐟brain/muscle
#IJMS 2025
www.mdpi.com/1422-0067/23...
Happy to share our work on the role of biomechanical PULSATILITY on lung vessels.
Following surgery to alleviate overload in kids with single ventricle hearts, lung arteries form AVMs. #DevBio #EndothelialCell #CHD
Beautiful work from Steve Spurgin. #DevBio 🫁 🫀 ❤️🩹
www.biorxiv.org/content/10.1...
"Plug"
Microglia (~20%) physically contract with #EndothelialCell & #Pericyte through gaps b/w adjacent #Astrocyte endfeet
A meticulous analysis of #MICrONS volumetric EM data
1 mm3 🐭visual cortex🤩
@gazz4science.bsky.social @sorengrubb.bsky.social #JCBFM 2024
journals.sagepub.com/doi/10.1177/...
AngioTag
Zebrafish kdrl+ #EndothelialCell RiboTag + TRAP-seq track endothelial translation activity in vivo
Discover novel EC functional genes😎
Profile Orangotypic endothelial features in🐟
Also UAS::RiboTag for any🐟tissues
Brant Weinstein lab #Angiogenesis 2025
link.springer.com/article/10.1...
#EndothelialCell GTPBP3, a mitochondrial enzyme in tRNA processing, ⏫#Angiogenesis in🐭embryo/retina/ischemic limb
Depending on mTORC1, not VEGFR/STAT/HIF1/NFκB
siGTPBP3▶️
⏫HRI▶️
⏫p-eIF2α/ATF4▶️
⏫Senstrin 2▶️
⏬mTORC1 activity
Also ⏫mitoROS ⏬OXPHOS
#Angiogenesis 2025
link.springer.com/article/10.1...
