Latest posts tagged with #cas9 on Bluesky
#IscB: The "Pocket-Sized" Future of Plant #GenomeEditing 📖✨
Evolutionary ancestors are making a comeback! Scientists have optimized IscB—the hypercompact predecessor of #Cas9 —for high-efficiency work in Rice.
Read the full paper: link.springer.com/article/10.1...
1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵
🔗 www.nature.com/articles/s41...
#CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics
Multiplex #CRISPR library for #Arabidopsis
2 #sgRNAs/construct → 3.03 genes/vector → 84.2% editing (vs. 48.4%)
Tissue-specific #Cas9 (5 promoters). Barcode tracking: 1,014 plants, 4.16 plants/gene.
SWEET11-14 quad KO → novel phenotype
CRISPys + MOFF + GuideMap
📄 doi.org/10.1016/j.ce...
🚀 We're excited to announce that our new issue is now online!
5️⃣ Featuring: 2 articles honouring #WomenInScience, a state-of-the-art Review, a Viewpoint, an Editor's Choice with a Commentary, several original articles.
Discover ➡️https://buff.ly/XIqf1Na
#lipidation #Cas9 #ubiquitin #structuralbiology
🦠 100× boost in algae #CRISPR #knockout via dsNHO "decoys"
Co-delivery of 24bp non-homologous DNA sequesters KU proteins → forces MMEJ pathway → 0.29% → 44.5% efficiency in Chlamydomonas.
Works with #Cas9 & #Cas12a.
Read the full study:
onlinelibrary.wiley.com/doi/10.1111/...
✂️🪲Injection-based insect genome editing: fusing a HUH endonuclease tag to #Cas9 boosts both #NHEJ ‑driven knockout and HDR‑mediated #knock‑in across multiple arthropods.
www.nature.com/articles/s42...
Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery.
Read more & apply here: bioscience.fi/events/crisp...
#geneediting #crispr #cas9
🧬 CRISPR technology is transforming genetics 🔬, enabling precise gene editing for research, diagnostics, and future therapeutic innovations 🚀.
#CRISPR #GeneEditing #Biotechnology #MolecularBiology #Cas9 #Genomics #LifeScienceStudents #ResearchInnovation #BiotechTrends #BioResire
The #FreeToRead #EditorsChoice article for the issue investigated the influence of IR25a on #electrophysiology and reproductive #behaviour in the #OrientalFruitFly, #Bactrocera dorsalis.
Why not give it a read?
doi.org/10.1111/imb.70005
#PestManagementStrategies #CRISPR #Cas9
A novel technology developed in the laboratory of Nobel Prize laureate Jennifer Doudna promises to once again revolutionize in vivo genome editing and gene therapy approaches #genetherapy #geneediting #Cas9
Sometimes #Cas9 is too dang big. But mini Cas proteins are bad at both NHEJ & HDR. PhD student Fedor Gorbenko used mammalian cell evolution for *HDR* to make REALLY super #Cas12f1 and #TnpB. As good as or better than Cas9! Base editors included! www.biorxiv.org/cgi/content/short/2025.10.27.684765v1
Checkout #SignalchemDx comprehensive portfolio of #CRISPR-associated #proteins inc highly active enzymes of exceptional specificity efficiency & versatility for the development of innovative & reliable CRISPR-based gene manipulation techniques
🔗hhttps://tinyurl.com/582d6akz
#CAS9 #genomics
#ForestryRes
Unlocking larch genome with CRISPR/Cas9 🌱🔬 - DNA-free, precise, and efficient. No more genomic integration worries! 🚀 Ready for conifer revolution.
Details: www.maxapress.com/article/doi/10.48130/for...
#crispr #cas9 #genome #dna
Modified Cas9 Proteins Cut Prime-Editing Errors by Up to Six‑Fold
MIT engineers created a modified Cas9 variant that cuts prime‑editing errors from about one in seven edits to roughly one in 101 in standard workflows, and to one in 543 in high‑precision mode. getnews.me/modified-cas9-proteins-c... #geneediting #cas9
#CRISPR study reveals surprising role of #Cas9 as guardian of #bacterial defense ...
| #bacteria | #virus | #phage | #spacers | #DNA | #RNA | By @umich.edu via @sciencex.bsky.social
🚀New from our lab @biorxivpreprint.bsky.social!
We reveal a cryptic metal binding pocket that dinamically forms in #CRISPR-Cas9 and regulates the metal-dependent activity of #Cas9.
#MarkovModels & #QuantumChemistry corroborated by biochem & #NMR experiments.
www.biorxiv.org/content/10.1...
Say hello to CATS, our new #bioinformatic tool that automates the comparison of #Cas9 nucleases and identification of #allele-specific #targets for mutations, making #CRISPR-Cas9 design faster and more precise!
GitHub: github.com/Physics4Medi...
Paper: www.frontiersin.org/journals/gen...
New research in #RESInsectMolecBio
The homeobox gene Distal-less is critical for the development of adult body appendages in #Bombyx mori
doi.org/10.1111/imb.13015
#InsectDevelopment #CRISPR #Cas9 #RNAseq
@sassanasgari.bsky.social @gulianusslab.bsky.social @wiley.com
3/ Too much #Cas9? You risk toxic effects and off-target cutting. Too little? Your editing efficiency plummets.
A novel rapid, sensitive, cost-effective, and non-radioactive method for Cas9 gRNA detection using a fluorophore-labeled DNA oligo.
Watch this video to learn more as Dr. Hu and his team explain the methodology.
Full-text here: doi.org/10.1016/j.ge...
#CRISPR #Cas9 #GeneEditing
Kevin Wasko (left) and Honglue Shi in front of their gel imager, confirming that the SpRY Cas9 variant struggles to effectively open DNA
“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – Co-first author Honglue Shi of the @doudna-lab.bsky.social on his recent paper in Molecular Cell
Read here: innovativegenomics.org/uncovering-c...
Impressive spacer selection mechanism by Cas9-adaptation supercomplexes - all pathway captured by #cryoEM #Cas9 🧬🧩🧵
The function of the ring-shaped protein Csn2 is reminiscent of the sliding clamp in DNA replication. Repurposing of #Cas9 nuclease by the #CRISPR adaptation machinery for prespacer selection characterized here demonstrates #Cas9 plasticity.
Upon PAM recognition the DNA-scanning supercomplex adopts the 'docked' conformation. Its further rearrangement via displacement of #Cas9 REC2 domain yields the 'locked' supercomplex with straight DNA.
Here, we present #cryoEM structures of the type II-A prespacer selection supercomplex consisting of #Cas9 RNP, Cas1-Cas2 #integrase fragment and Csn2 ring in the DNA-scanning and two different PAM-bound configurations.
In type II-A #CRISPR-Cas systems acquisition of new spacers involves the effector nuclease #Cas9, which forms a ‘supercomplex’ with the Cas1-Cas2 #integrase and the ring-shaped Csn2 protein. The supercomplex structure and the role of Csn2 remain unknown.
The next speaker is selected from the submitted abstracts and is Deja Porenta who is working on assessing the Cas9-directed immune response in mice at the @utrechtuniversity.bsky.social #NVGCT2025 #LNPs #Cas9 #nanoparticles
#CRISPR capable of even more than we thought ...
| #Bacteria | #Cas9 | #virus | #RNA | By @rockefelleruniv.bsky.social via @eurekalert.bsky.social
In the last couple of decades, scientists revealed many details on the microbial #ImmuneSystem which protects them against their own #pathogens, like #phages, for example. One of the unexpected outcomes of these discoveries is the development of the #CRISPR - #Cas9 system used for #GeneEditing.
Noor Al-Sayyad
“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – @honglue.bsky.social of the @doudna-lab.bsky.social on his paper in Molecular Cell.
Read here: shorturl.at/RoKgG
Pictured: Co-first author Noor Al-Sayyad
